Core Co-leader: Asish Chaudhuri, PhD
Compromised mitochondrial function and accumulation of oxidative damage to cell components with age have been proposed as primary factors underlying age-associated alterations in physiologic function and pathology.
The Oxidative Damage and Mitochondrial Function Core provides investigators with reliable and sensitive methods to measure markers of oxidative damage in lipids, protein, and DNA in biological samples and reliable, standardized, state-of-the-art assays of mitochondrial function. Many of these assays utilize expensive and sophisticated equipment that require significant expertise for performance and maintenance. Thus, a primary strength of the Core is our ability to offer precise, reproducible and standardized assays conducted by experienced Core personnel using sophisticated equipment that may not be readily available in an individual investigator's laboratory.
- Measurement of F2-isoprostanes, a marker of lipid peroxidation in plasma, tissues, or urine by GC-MS.
- Measurement of oxidative damage to DNA using HPLC-based analysis of oxo-8dG.
- Measurements of oxidative damage and modification to proteins using three complementary sets of sensitive and reliable assays for quantitative assessments of the oxidation states of proteins (i) protein carbonylation (ii) disulfide formation, and (iii) alteration of protein surface hydrophobicity.
- Assays of mitochondrial function include the use of direct, sensitive measurements of H2O2release from isolated mitochondria, mitochondrial superoxide generation using EPR, and mitochondrial function assays in cells using a relatively new technology provided by Seahorse Biosciences that allows direct measurement of mitochondrial function in cells without requiring isolation of mitochondria.
Services are partially underwritten by the NIA Nathan Shock Center. Contact the Core Leader or the specific Core Co-leader for details regarding your assay of interest.