Contact

Location: STRF 2.292.5

Department

Medicine

Xianlin Han, PhD

Professor

Personal Statement:

I am an internationally-renowned investigator in the fields of lipidomics, lipid metabolism, and lipid biochemistry. I have developed expertise in broad areas of research, such as diabetes, neuroscience, and metabolic biochemistry. The mass spectrometric techniques for lipidomics developed in our group, collectively termed “multi-dimensional mass spectrometry-based shotgun lipidomics” with unparalleled high sensitivity and comprehensive coverage, have been widely used to identify altered lipid metabolism, trafficking, and homeostasis, and biomarkers under patho(physio)logical conditions. The current interests of my laboratory research focus on diabetic neuropathy, mitochondrial dysfunction in diabetic hearts, and Alzheimer’s disease. I have the expertise, leadership, training, and motivation necessary to conduct these research projects. As an investigator or co-investigator on over twenty previous NIH-funded grants, I have successfully administered the projects and produced several peer-reviewed publications from each project, resulting in a total of over 250 peer-reviewed publications. It is gratifying that my contribution has received over 21,000 citations (over half are since 2013) with an h-index of 72 (Google Scholar, 2018).


Education

Year Degree Discipline Institution
1982 BS Chemistry Zhejiang University
P.R. , China
1985 MS Chemical Thermodynamics Zhejiang University
P.R. , China
1987 MA Physical Chemistry Washington University
St. Louis , MO
1990 Internship Washington University
St. Louis , MO
1990 PhD Biophysical and Bioanalytical Chemistry Washington University
St. Louis , MO

Research

Our research focuses on identification of the molecular and biochemical mechanisms underlying alterations in lipid metabolism, signaling, and homeostasis that occur under patho(physio)logical conditions such as aging, Alzheimer’s disease (AD), diabetes, and cancer by lipidomics. Lipid metabolism spans a highly elaborate system comprised of numerous classes and subclasses, and hundreds of thousands of species that make up the cellular lipidomes. A large number of pathways and networks which are highly dynamic and interwoven are involved in lipid metabolism. Identifying the mechanisms underpinning alterations in lipid metabolism, signaling, and homeostasis that occur under patho(physio)logical conditions could unravel disease pathogenesis, uncover drug targets for treatment, and identify biomarkers for early diagnosis and prognosis of the diseases. Lipidomics, which facilitates large-scale analysis of cellular lipidomes based on the principles and techniques of analytical chemistry, could allow us to comprehensively and effectively determine alterations in lipid metabolism, signaling, and homeostasis under the conditions.

Our laboratory is one of the world-leading research groups on lipidomics. We have developed an enabling technology with in-house software programs termed “multi-dimensional mass spectrometry-based shotgun lipidomics (MDMS-SL)”, initiated in the early 1990s and still under constant evolution. The MDMS-SL technology provides modular, robust, and label-free quantification of lipids. At its current stage, the technology enables us to identify and quantify over 50 lipid classes, over 95% of lipid mass content, and thousands of individual lipid molecular species from limited amounts of biological samples in an accurate (>90% reproducibility) and relatively high throughput fashion. By using lipidomics, we have developed a few research projects (supported with NIH funds and other sources) including AD and diabetes-associated dementia, as well as multiple minor translational research projects such as anesthetics-induced neurotoxicity and cancer biomarkers. By using our enabling lipidomics technology, we have also established numerous (inter)national collaborations on a variety of research areas.

Awards & Accomplishments

2003 Memory Ride Prize
2017-Present Methodist Hospital Foundation Chair in Aging Studies and Research, University of Texas Health Science Center at San Antonio
2018 University of Texas System STARs Award
Guest Professors Changchun Institute of Applied Chemistry, Chinese Academy of Sciences; Soochow University; and Oil Crop Research Institute, Chinese Academy of Agricultural Sciences

Affiliations

Barshop Institute for Longevity and Aging Studies
Glenn Biggs Institute for Alzheimer’s & Neurodegenerative Diseases
Department of Medicine, Division of Diabetes
Department of Biochemistry and Structural Biology
University of Texas Health Science Center at San Antonio

Lab Members

Dr. Chunyan Wang Senior Post-Doctoral Fellow

Dr. Juan Pablo Palavicini
Senior Post-Doctoral Fellow

Dr. Jianing Wang
Post-Doctoral Fellow
Dr. Shulan Qiu Post-Doctoral Fellow

Mr. Charles Chao Qin
Research Associate
Prof. Juan Chen Visiting Scholar
Prof. Zhenli Min Visiting Scholar

News

Dr. Han is the University of Texas System STARs Awardee.
Drs. Han and Seshadri received ~$3.5 million grant award from the NIH National Institute on Aging to unravel the mechanisms by which APOE2 confers neuroprotection against aging and AD.

Publications

Analytical challenges of shotgun lipidomics at different resolution of measurements.
Wang J, Han X
TrAC Trends in Analytical Chemistry. 2019 Oct 17. doi: 10.1016/j.trac.2019.115697. [In press]

Can Oral Health and Oral-derived Biospecimens Predict Progression of Dementia?
Orr ME, Reveles KR, Yeh CK, Young EH, Han X
Oral Diseases. 2019 Sep 21. doi: 10.1111/odi.13201. [Epub ahead of print]
PMID: 31541581

Loss of mitochondrial energetics is associated with poor recovery of muscle function but not mass following disuse atrophy.
Trevino MB, Zhang X, Standley RA, Wang M, Han X, Reis FCG, Periasamy M, Yu G, Kelly DP, Goodpaster BH, Vega RB, Coen PM
American Journal of Physiology. Endocrinology and Metabolism. 2019 Sep 3. doi: 10.1152/ajpendo.00161.2019. [Epub ahead of print]
PMID: 31479303

Novel strategies for enhancing shotgun lipidomics for comprehensive analysis of cellular lipidomes.
Hu C, Wang C, He L, Han X
TrAC Trends in Analytical Chemistry. 2018 Nov 27. doi: doi.org/10.1016/j.trac.2018.11.028. [In press]

Tutorial on lipidomics.
Wang J, Wang C, Han X
Analytica Chimica Acta. 2019 Jul 11;1061:28-41. doi: 10.1016/j.aca.2019.01.043. Epub 2019 Feb 1.
PMID: 30926037

Strategies to Improve/Eliminate the Limitations in Shotgun Lipidomics.
Hu C, Duan Q, Han X
Proteomics. 2019 Jul 10:e1900070. doi: 10.1002/pmic.201900070. [Epub ahead of print]
PMID: 31291508

MondoA drives muscle lipid accumulation and insulin resistance.
Ahn B, Wan S, Jaiswal N, Vega RB, Ayer DE, Titchenell PM, Han X, Won KJ, Kelly DP
JCI Insight. 2019 Jul 9;5. pii: 129119. doi: 10.1172/jci.insight.129119.
PMID: 31287806

Association of Altered Liver Enzymes With Alzheimer Disease Diagnosis, Cognition, Neuroimaging Measures, and Cerebrospinal Fluid Biomarkers.
Nho K, Kueider-Paisley A, Ahmad S, MahmoudianDehkordi S, Arnold M, Risacher SL, Louie G, Blach C, Baillie R, Han X, Kastenmüller G, Trojanowski JQ, Shaw LM, Weiner MW, Doraiswamy PM, van Duijn C, Saykin AJ, Kaddurah-Daouk R; Alzheimer’s Disease Neuroimaging Initiative and the Alzheimer Disease Metabolomics Consortium.
JAMA Network Open. 2019 Jul 3;2(7):e197978. doi: 10.1001/jamanetworkopen.2019.7978.
PMCID: PMC6669786

Dynamic changes to lipid mediators support transitions among macrophage subtypes during muscle regeneration.
Giannakis N, Sansbury BE, Patsalos A, Hays TT, Riley CO, Han X, Spite M, Nagy L
Nature Immunology. 2019 May;20(5):626-636. doi: 10.1038/s41590-019-0356-7. Epub 2019 Apr 1.
PMCID: PMC6537107

AMP-activated protein kinase activation ameliorates eicosanoid dysregulation in high-fat-induced kidney disease in mice.
Declèves AE, Mathew AV, Armando AM, Han X, Dennis EA, Quehenberger O, Sharma K
Journal of Lipid Research. 2019 May;60(5):937-952. doi: 10.1194/jlr.M088690. Epub 2019 Mar 12.
PMCID: PMC6495162 [Available on 2020-05-01]

Defective Phosphatidylglycerol Remodeling Causes Hepatopathy, Linking Mitochondrial Dysfunction to Hepatosteatosis.
Zhang X, Zhang J, Sun H, Liu X, Zheng Y, Xu D, Wang J, Jia D, Han X, Liu F, Nie J, Shi Y
Cellular and Molecular Gastroenterology and Hepatology. 2019;7(4):763-781. doi: 10.1016/j.jcmgh.2019.02.002. Epub 2019 Mar 1.
PMCID: PMC6463126

Phosphatidylethanolamine made in the inner mitochondrial membrane is essential for yeast cytochrome bc1 complex function.
Calzada E, Avery E, Sam PN, Modak A, Wang C, McCaffery JM, Han X, Alder NN, Claypool SM
Nature Communications. 2019 Mar 29;10(1):1432. doi: 10.1038/s41467-019-09425-1.
PMCID: PMC6441012

Hepatocyte-Macrophage Acetoacetate Shuttle Protects against Tissue Fibrosis.
Puchalska P, Martin SE, Huang X, Lengfeld JE, Daniel B, Graham MJ, Han X, Nagy L, Patti GJ, Crawford PA
Cell Metabolism. 2019 Feb 5;29(2):383-398.e7. doi: 10.1016/j.cmet.2018.10.015. Epub 2018 Nov 15.
PMCID: PMC6559243 [Available on 2020-02-05]

Altered bile acid profile in mild cognitive impairment and Alzheimer’s disease: Relationship to neuroimaging and CSF biomarkers.
Nho K, Kueider-Paisley A, MahmoudianDehkordi S, Arnold M, Risacher SL, Louie G, Blach C, Baillie R, Han X, Kastenmüller G, Jia W, Xie G, Ahmad S, Hankemeier T, van Duijn CM, Trojanowski JQ, Shaw LM, Weiner MW, Doraiswamy PM, Saykin AJ, Kaddurah-Daouk R; Alzheimer’s Disease Neuroimaging Initiative and the Alzheimer Disease Metabolomics Consortium.
Alzheimer’s & Dementia. 2019 Feb;15(2):232-244. doi: 10.1016/j.jalz.2018.08.012. Epub 2018 Oct 15.
PMCID: PMC6454538

Synthesis, preliminarily biological evaluation and molecular docking study of new Olaparib analogues as multifunctional PARP-1 and cholinesterase inhibitors.
Gao CZ, Dong W, Cui ZW, Yuan Q, Hu XM, Wu QM, Han X, Xu Y, Min ZL
Journal of Enzyme Inhibition and Medicinal Chemistry. 2019 Dec;34(1):150-162. doi: 10.1080/14756366.2018.1530224.
PMCID: PMC6237161

Altered bile acid profile associates with cognitive impairment in Alzheimer’s disease-An emerging role for gut microbiome.
MahmoudianDehkordi S, Arnold M, Nho K, Ahmad S, Jia W, Xie G, Louie G, Kueider-Paisley A, Moseley MA, Thompson JW, St John Williams L, Tenenbaum JD, Blach C, Baillie R, Han X, Bhattacharyya S, Toledo JB, Schafferer S, Klein S, Koal T, Risacher SL, Kling MA, Motsinger-Reif A, Rotroff DM, Jack J, Hankemeier T, Bennett DA, De Jager PL, Trojanowski JQ, Shaw LM, Weiner MW, Doraiswamy PM, van Duijn CM, Saykin AJ, Kastenmüller G, Kaddurah-Daouk R; Alzheimer’s Disease Neuroimaging Initiative and the Alzheimer Disease Metabolomics Consortium.
Alzheimer’s & Dementia. 2019 Jan;15(1):76-92. doi: 10.1016/j.jalz.2018.07.217. Epub 2018 Oct 15.
PMCID: PMC6487485 [Available on 2020-01-01]

Lipidomics reveals a systemic energy deficient state that precedes neurotoxicity in neonatal monkeys after sevoflurane exposure.
Wang C, Liu F, Frisch-Daiello JL, Martin S, Patterson TA, Gu Q, Liu S, Paule MG, Hanig JP, Slikker W Jr, Crawford PA, Wang C, Han X
Analytica Chimica Acta. 2018 Dec 11;1037:87-96. doi: 10.1016/j.aca.2017.11.052. Epub 2017 Nov 30.
PMID: 30292318

Hepatic ketogenic insufficiency reprograms hepatic glycogen metabolism and the lipidome.
d’Avignon DA, Puchalska P, Ercal B, Chang Y, Martin SE, Graham MJ, Patti GJ, Han X, Crawford PA
JCI Insight. 2018 Jun 21;3(12). pii: 99762. doi: 10.1172/jci.insight.99762. [Epub ahead of print]
PMCID: PMC6124396

Enhanced coverage of lipid analysis and imaging by matrix-assisted laser desorption/ionization mass spectrometry via a strategy with an optimized mixture of matrices.
Wang J, Wang C, Han X
Analytica Chimica Acta. 2018 Feb 13;1000:155-162. doi: 10.1016/j.aca.2017.09.046. Epub 2017 Oct 17.
PMID: 29289304

Strategy for Quantitative Analysis of Isomeric Bis(monoacylglycero)phosphate and Phosphatidylglycerol Species by Shotgun Lipidomics after One-Step Methylation.
Wang M, Palavicini JP, Cseresznye A, Han X
Analytical Chemistry. 2017 Aug 15;89(16):8490-8495. doi: 10.1021/acs.analchem.7b02058. Epub 2017 Jul 26.
PMID: 28708380